

簡要描述:Hams F12 Medium (產(chǎn)品僅供科研參考) 型號: ......
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Ham's F12 Medium 是根據(jù) Richard G. Ham 于 1965 年報(bào)告的液體合成培養(yǎng)液配方,經(jīng)過我們公司特殊的制備方法調(diào)制而成的粉末培養(yǎng)基。
該培地非常適用于哺乳動物細(xì)胞的單細(xì)胞培養(yǎng),并廣泛用于 CHO 細(xì)胞的培養(yǎng)。此外,它還表現(xiàn)出在人類細(xì)胞以及各種細(xì)胞的原代培養(yǎng)中具有優(yōu)良的增殖支持能力。
稱取 10.6g 該粉末培養(yǎng)基溶解于蒸餾水中,制成 1000mL 溶液。在此過程中,溫度不要超過 37 攝氏度。加入適量(加入 1.2-2.0g 后,在 5% CO? 下,37℃時(shí) pH 值為 7.1-7.4),溶解后立即進(jìn)行過濾滅菌。預(yù)先使用 CO? 氣體調(diào)節(jié) pH 值后再進(jìn)行過濾可以防止過度的 pH 升高。如不立即使用,應(yīng)密封保存在陰涼處(2-10℃)。在使用前,根據(jù)需要加入適量的血清。
該粉末培養(yǎng)基需要密封保存,并放置在干燥陰涼避光處(2-10℃)。
生產(chǎn)之后一年。
每 10.6g (1 L)
Sodium Chloride................................ 7,599 mg
Potassium Chloride............................. 224 mg
Calcium Chloride(Anhydrate).................. 33.31 mg
Magnesium Chloride (Hexahydrate)............. 122 mg
Disodium Hydrogen Phosphate(Dihydrate)...... 177.97 mg
Copper Sulfate (II) (Pentahydrate)............. 0.0025 mg
Zinc Sulfate(Heptahydrate)..................... 0.863 mg
Iron(II)Sulfate(Heptahydrate).................. 0.834 mg
Glucose.......................................... 1,802 mg
L-Arginine Hydrochloride...................... 211 mg
L-Alanine....................................... 8.91 mg
L-Asparagine(Monohydrate).................... 15.01 mg
L-Aspartic Acid............................... 13.31 mg
L-Cysteine Hydrochloride Hydrate.............. 35.12 mg
L-Glutamic Acid............................... 14.71 mg
L-Glutamine................................... 146 mg
Glycine......................................... 7.51 mg
L-Histidine Hydrochloride Hydrate............ 20.96 mg
L-Isoleucine................................... 3.94 mg
L-Leucine...................................... 13.12 mg
L-Lysine Hydrochloride........................ 4.48 mg
L-Phenylalanine............................... 4.96 mg
L-Proline....................................... 34.53 mg
L-Serine........................................ 10.51 mg
L-Threonine..................................... 11.91 mg
L-Tryptophan.................................... 2.04 mg
L-Tyrosine...................................... 5.44 mg
L-Valine........................................ 11.71 mg
D-Biotin........................................ 0.0073 mg
Choline Chloride.............................. 14 mg
Calcium Pantothenate.......................... 0.477 mg
Folic Acid...................................... 1.32 mg
Hypoxanthin................................... 4.08 mg
Myo-Inositol................................... 18 mg
Putrescine Dihydrochloride.................... 0.161 mg
Pyridoxine Hydrochloride...................... 0.062 mg
Riboflavin...................................... 0.038 mg
Sodium Pyruvate............................... 110 mg
Thiamine Hydrochloride........................ 0.377 mg
Thymidine....................................... 0.727 mg
Cyanocobalamin............................... 1.36 mg
Nicotinamide................................... 0.037 mg
α-Lipoic Acid................................... 0.206 mg
Linoleic Acid................................... 0.084 mg
Phenol Red...................................... 1.24 mg
Other substances to be added
Sodium Bicarbonate.......................... As Needed
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